Z-Active Recombinant Cas9 protein

The CRISPR/Cas9 system is a powerful tool for gene editing which transform research at an astonishing rate. To achieve maximum editing efficiency across a broad spectrum of cell types, including iPSCs and primary cells, we’ve refined and optimized rate-limiting components of CRISPR/Cas9 system. Our Z-Active^TM Recombinant Cas9 protein (Electroporation-Ready) was purified from E. coli. utilized folding optimization strategies which maximized Cas9 enzyme activity by achieving functional knockout in up to 98% of primary hematopoietic stem cells (HSCs) (Figure 1).

High editing efficiency without further selection after transfection is especially important when working with difficult-to-edit genomic loci and cells more resistant to protein or DNA delivery such as primary cells, immune cells, and stem cells. we were able to demonstrate over 2 fold higher editing efficiency of easy-to-edit chromosome regions, and more than 2 to 5 fold higher editing efficiency of difficult-to-edit genomic loci than competitors in the market for functional knockout of genes in donor-derived primary cell populations (Figure 1&2).