🧫 Organoid Culture Solutions

High-Bioactivity Proteins for Organoid Culture

FDA Modernization Act 2.0 ready — Z-Active® tagless cytokines and growth factors with 5–10× higher bioactivity for next-generation organoid models. HEK293 mammalian expression with chaperone-assisted folding ensures proper glycosylation and disulfide bonding critical for organoid self-organization.

Browse Catalog Contact Sales
Core Signaling Pathways
Wnt BMP FGF EGF TGF-β HGF Neurotrophins

Organoid Types & Required Growth Factors

🟢 = Z-Active® catalog protein (SKU listed)  |  ⚪ = Inquire for availability

🧠

Brain

BR
🟢 Noggin (RP490) 🟢 FGF2 (RP471) 🟢 EGF (RP480) 🟢 GDNF (RP458) 🟢 BMP4 (RP898) 🟢 DKK1 (RP931) 🟢 SHH (RP612) ⚪ BDNF
🫁

Liver

LV
🟢 Activin A (RP896) 🟢 Noggin (RP490) 🟢 R-Spondin-1 (RP618) 🟢 Wnt3a (RP895) 🟢 EGF (RP480) 🟢 HGF (RP430) 🟢 OSM (RP440) 🟢 BMP4 (RP898) 🟢 FGF10 (RP902)
🔬

Intestine

IN
🟢 Activin A (RP896) 🟢 R-Spondin-1 (RP618) 🟢 Noggin (RP490) 🟢 Wnt3a (RP895) 🟢 EGF (RP480) 🟢 IGF1 (RP540)
💡 WENR: RP895+RP480+RP490+RP618
🫘

Kidney

KD
🟢 Activin A (RP896) 🟢 Noggin (RP490) 🟢 R-Spondin-1 (RP618) 🟢 BMP4 (RP898) 🟢 BMP7 (RP899) 🟢 FGF2 (RP471)
🌬️

Lung

LG
🟢 Activin A (RP896) 🟢 Noggin (RP490) 🟢 R-Spondin-1 (RP618) 🟢 Wnt3a (RP895) 🟢 FGF7 (RP470) 🟢 BMP4 (RP898)
🟡

Pancreas

PC
🟢 Noggin (RP490) 🟢 R-Spondin-1 (RP618) 🟢 Wnt3a (RP895) 🟢 EGF (RP480) 🟢 FGF10 (RP902)
🟠

Stomach

ST
🟢 Activin A (RP896) 🟢 Noggin (RP490) 🟢 R-Spondin-1 (RP618) 🟢 Wnt3a (RP895) 🟢 EGF (RP480) 🟢 BMP4 (RP898) 🟢 FGF10 (RP902)
🔵

Prostate

PR
🟢 Noggin (RP490) 🟢 EGF (RP480) 🟢 R-Spondin-1 (RP618) 🟢 BMP4 (RP898) 🟢 FGF2 (RP471)
🦋

Thyroid

TH
🟢 Activin A (RP896) 🟢 Noggin (RP490) 🟢 Wnt3a (RP895) 🟢 EGF (RP480) 🟢 BMP4 (RP898) 🟢 FGF2 (RP471)
❤️

Vascular

VA
🟢 Activin A (RP896) 🟢 BMP4 (RP898) 🟢 VEGFA (RP460) 🟢 PDGFD (RP954) 🟢 FGF2 (RP471)
👁️

Retinal

RT
🟢 Noggin (RP490) 🟢 DKK1 (RP931) 🟢 IGF1 (RP540) 🟢 SHH (RP612) 🟢 FGF2 (RP471)
🩷

Endometrium

EM
🟢 Wnt3a (RP895) 🟢 R-Spondin-1 (RP618) 🟢 EGF (RP480) 🟢 HGF (RP430) 🟢 IL6 (RP260) 🟢 FGF2 (RP471)
🎯

Tumor PDO

PDO
🟢 EGF (RP480) 🟢 Noggin (RP490) 🟢 R-Spondin-1 (RP618) 🟢 Wnt3a (RP895) 🟢 FGF10 (RP902) 🟢 HGF (RP430)

Organoid Protein Bundles

Purpose-built protein combinations with pathway rationale — optimized for specific organoid workflows.

⚡ BUNDLE

Intestinal Organoid Bundle — WENR-MAX

ORG-WENR-MAX · RP895 · RP480 · RP490 · RP618
Target: Intestinal, stomach, pancreatic, and tumor PDO organoids — the classic WENR cocktail.

Essential for Lgr5⁺ stem-cell–driven organoid cultures. WENR-MAX combines Wnt3a, EGF, Noggin, and R-Spondin 1 to sustain self-renewal, maintain crypt-villus architecture, and support long-term passaging.

Components & Pathways

  • Wnt3a: canonical Wnt signaling — stem cell self-renewal
  • EGF (EGFR): MAPK/PI3K proliferation & epithelial expansion
  • Noggin: BMP antagonist — maintains stemness
  • R-Spondin 1: Wnt potentiation via Lgr5 receptor
Wnt3a (RP895) EGF (RP480) Noggin (RP490) R-Spondin-1 (RP618)
$1,760.00 (50μg each component)
View Bundle & Add to Cart
⚡ BUNDLE

Hepatic Organoid Bundle — HEPATO-CORE

ORG-HEP-CORE · RP896 · RP430 · RP480
Target: Human hepatic organoids / liver epithelial 3D models — expansion, maturation, function-forward assays.

Judge cultures by metabolic competence, not growth alone. HEPATO-CORE combines HGF, EGF, and FGF19 to support receptor-driven expansion while biasing workflows toward CYP450 activity, albumin secretion, and metabolic readouts alongside your base medium.

Components & Pathways

  • HGF (c-Met): mitogenesis, morphogenesis, pro-survival programs
  • EGF (EGFR): epithelial expansion & post-split recovery
  • FGF19: FGFR4-biased liver contexts; mature-like hepatocyte traits (protocol-dependent)
Activin A (RP896) HGF (RP430) EGF (RP480)
$1,080.00 (50μg each component)
View Bundle & Add to Cart
⚡ BUNDLE

Tumor Bundle — CRC-PDO MAX

ORG-CRC-PDO · RP895 · RP480 · RP490 · RP618
Target: Colorectal patient-derived organoids (PDOs) & Wnt-high tumor epithelial cultures.

Balance stem-like propagation against stress/differentiation programs. CRC-PDO MAX combines EGF with Wnt3a, R-Spondin1, Noggin, plus a protocol-appropriate TGF-β inhibitor module for many establishment/expansion media designs — preserving interpretable drug sensitivity windows.

Components & Pathways

  • EGF: MAPK/PI3K-linked proliferation & recovery
  • Wnt3a + R-Spondin1 + Noggin: canonical Wnt potentiation + BMP restraint
  • TGF-β inhibitor: small-molecule module (non-protein) where indicated
Wnt3a (RP895) EGF (RP480) Noggin (RP490) R-Spondin-1 (RP618)
$1,760.00 (50μg each component)
View Bundle & Add to Cart
⚡ BUNDLE

Tumor Bundle — PDAC-STRESS SHIELD

ORG-PDAC · RP480 · RP490 · RP470
Target: Pancreatic ductal adenocarcinoma (PDAC) organoids — chemoresistance & therapy profiling.

PDAC cultures are sensitive to mechanical stress and establishment dropout. PDAC-STRESS SHIELD pairs EGF, FGF10, and Noggin with a ROCK inhibitor module (small molecule) for protocols using transient Rho/ROCK relief — improving survival/cloning while keeping stimulation defined.

Components & Pathways

  • EGF: proliferation / survival
  • FGF10: ductal-adjacent programs common in PDAC media
  • Noggin: BMP restraint
  • ROCK inhibitor: small-molecule module (non-protein) where indicated

Applications

Chemoresistance (pulse-chase, DNA damage recovery); combination cytotoxic + targeted screens; baseline epithelial models before stroma co-culture.

EGF (RP480) FGF10 (RP902) Noggin (RP490)
$980.00 (50μg each component)
View Bundle & Add to Cart
⚡ BUNDLE

Neural Organoid Bundle — NEURO-PATTERN+

ORG-NEURO · RP471 · RP480 · RP458
Target: Brain-region neural organoids & NPC-derived 3D cultures — patterning & maturation.

Neural organoids fail quietly: lineage skew, stalled maturation, premature exhaustion. NEURO-PATTERN+ bundles Wnt pathway regulators, BMP pathway regulators, BDNF, and GDNF to clarify axis control ahead of standard small-molecule patterning switches in neural protocols.

Components & Pathways

  • Wnt regulators: progenitor/regionalization programs (protocol-dependent)
  • BMP regulators: boundary control (model-dependent)
  • BDNF / GDNF: TrkB/RET-linked survival, neurite outgrowth, maturation support

Applications

Neurodevelopmental disease modeling; neurodegeneration (proteostasis, stressors); long differentiation/toxicity studies.

FGF2 (RP471) EGF (RP480) GDNF (RP458)
🔬 Boston Molecules Advantage: Ultra-low endotoxin + high-specific-activity ligands protect week-scale runs where inflammation mimics disease biology.
🏷️ Tag-free here: Long runs integrate small biases; tag-free morphogens/neurotrophins reduce aggregation & non-specific binding drift.
$780.00 (50μg each component)
View Bundle & Add to Cart

Z-Active® Organoid Protein Catalog

Protein SKU Key Organoid Applications
Human Wnt3a RP895 Intestine, Liver, Pancreas, PDO, Stomach, Endometrium View
Human Activin A RP896 Liver, Intestine, Stomach, Kidney, Thyroid, Vascular View
Human EGF RP480 Intestine, Liver, Lung, Pancreas, PDO, Brain, Stomach View
Human Noggin RP490 WENR, Liver, Lung, Brain, Kidney, Retinal, Prostate View
Human R-Spondin 1 RP618 Intestine, Liver, Stomach, PDO, Lung, Endometrium View
Human HGF RP430 Liver maturation, Endometrium, Tumor PDO View
Human FGF2 (bFGF) RP471 Brain, Kidney, Prostate, Thyroid, Vascular, Retinal View
Human FGF7 (KGF) RP470 Lung proximal airway View
Human FGF10 RP902 Liver, Pancreas, Stomach, Tumor PDO View
Human BMP4 RP898 Brain, Kidney, Lung, Stomach, Prostate, Thyroid, Vascular View
Human BMP7 RP899 Kidney differentiation View
Human DKK1 RP931 Brain, Retinal (Wnt inhibitor) View
Human IGF1 RP540 Intestine, Retinal View
Human VEGFA RP460 Vascular organoids View
Human SHH RP612 Brain, Retinal (Hedgehog signaling) View
Human IL6 RP260 Endometrium View
Human PDGFD RP954 Vascular organoids View
Human GDNF RP458 Brain / neural maturation View
Human OSM RP440 Liver maturation View
⚖️

FDA Modernization Act 2.0

The FDA Modernization Act 2.0 (signed December 2022) eliminates the mandate for animal testing prior to human clinical trials. Drug developers can now use organoids, organ-on-chip, and other in vitro models as alternatives — accelerating the adoption of organoid technology across the pharmaceutical industry.

High-quality, bioactive growth factors are essential for organoid establishment and long-term maintenance. Z-Active® proteins — with their 5–10× higher bioactivity — enable more robust organoid formation, reduced growth factor consumption, and more reproducible results.

Need a Custom Organoid Panel?

Our scientists can help you design a custom cytokine and growth factor panel tailored to your specific organoid model. Contact us for bulk pricing and custom formulations.

Talk to a Scientist 📞 617-651-1016