Mouse CCL20/MIP-3A Recombinant Protein (HEK293 Expression) — Tag Free
Price range: $198.00 through $5,200.00
HEK293 × Chaperone-Assisted Technology
Produced using our proprietary Z-Active® platform — HEK293 mammalian expression with chaperone-assisted folding for proper disulfide bonding, N-linked glycosylation, and 5–10× higher bioactivity vs Leading Suppliers.
Overview
CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20 – 28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71 and 63% amino acid sequence homology with rat and human MIP-3 alpha, respectively.MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.
Specifications
- Species: mouse
- Expression System: HEK293
- Sequence: Ala27-Met96
- Tag: Tag Free
- Molecular Weight: 8.0 kDa
- Purity: > 95%, determined by SDS-PAGE
- Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method
- UniProt/Swiss-Prot: O89093
- Available Sizes: 10 µg, 50 µg, 1 mg
Bioactivity
Measured by its ability to chemoattract BaF3 mouse pro-B cells
transfected with human CCR6
Storage & Stability
Aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.
Quality Assurance
Each lot is tested for purity (SDS-PAGE, SEC-HPLC), endotoxin levels (LAL method), and bioactivity. A Certificate of Analysis (COA) is provided with every order.
Safety Data Sheets & Documentation
Protocols & Support
Reconstitution Protocol
1. Briefly centrifuge the vial to collect lyophilized powder at the bottom.
2. Reconstitute in sterile deionized water or PBS to a concentration of 0.2–1.0 mg/mL.
3. Gently pipette up and down to dissolve. Do not vortex.
4. Aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Download Data Sheet
Complete product data sheet including batch-specific QC data, sequence information, and storage guidelines.
Download PDF Data Sheet
