Human TGFb2 Recombinant Protein (HEK293 Expression) — Tag Free

Overview

TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta   superfamily that share a characteristic cysteine knot structure (1-7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines  proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial_x0002_mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 2 (8, 9). Disul_x001D_de-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 2 shows 100% aa identity with porcine, canine,  equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity (1). TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta  RIII) and a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates another ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Use of other signalin.

Specifications

• Species: human
• Expression System: HEK293
• Sequence: Ala303-Ser414.
• Tag: Tag Free
• Molecular Weight: 12.7 kDa
• Purity: ≥95%, determined by SDS-PAGE
• Endotoxin: ＜0.10 EU per 1 ug of the protein by the LAL method.
  Quality
  Solution protein.
  Dissolved in sterile 20 mM sodium citrate(pH=2.5) bu_x001F_er .
  This solution can be diluted into other aqueous bu_x001F_ers. Centrifuge the vial
  prior to opening.
  Formulation
  Storage and Stability
  Shipping Shipping with dry ice.
  Components and Storage
  Synonyms
  12.7 kDa (monomer).
  Accession #
  Predicted Mol Mass
  Source
  P61812.
  General Information
  kDa NR R
  Bioactivity
  4 ug/lane protein was resolved
  with SDS-PAGE under non-reducing (NR)
  and reducing (R)conditions and
  visualized by Coomassie blue staining.
  HEK293
  Ala303-Ser414.
  SDS-PAGE
  BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor
  factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2;
  TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2.
  105
  72
  60
  26
  37
  17
  50
  Activity Measured by the(CAGA)12-luciferase reporter assay.
  The EC50 for this e_x001F_ect is 0.69ng/mL
• UniProt/Swiss-Prot: P61812
• Available Sizes: 10 µg, 50 µg, 1 mg

Storage & Stability

Aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.

Quality Assurance

Each lot is tested for purity (SDS-PAGE, SEC-HPLC), endotoxin levels (LAL method), and bioactivity. A Certificate of Analysis (COA) is provided with every order.

Safety Data Sheets & Documentation

Protocols & Support