Human TGFb3 Recombinant Protein (HEK293 Expression) — Tag Free
Price range: $198.00 through $4,600.00
HEK293 × Chaperone-Assisted Technology
Produced using our proprietary Z-Active® platform — HEK293 mammalian expression with chaperone-assisted folding for proper disulfide bonding, N-linked glycosylation, and 5–10× higher bioactivity vs Leading Suppliers.
Overview
TGF-beta 3 (transforming growth factor-beta 3) is a member of a TGF-beta superfamily subgroup that is defined by their structural and functional similarities (1-5). TGF-beta 3 and its closely related proteins, TGF-beta 1 and –beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial–mesenchymal transition (4, 6, 7). The non-redundant biological effects of TGF-beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development (1, 2, 7-9). Human TGF–beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin-like convertase to generate a 220 aa latency-associated peptide (LAP) and a 112 aa mature TGF–beta 3 (10, 11). Mature human TGF–beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse , rat, and pig TGF–beta 3, respectively. TGF-beta 3 is secreted as a complex with LAP. This latent form of TGF-beta 3 becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin-1, and a subset of integrins (12). TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF–beta RI or ALK–1, to modulate transcription through Smad phosphorylation (13-15). The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF–beta RIII and endoglin) and the subsequent initiation of Smad–dependent or -independent signaling pathways (14, 16, 17).
Specifications
- Species: human
- Expression System: HEK293
- Sequence: Ala301-Ser412.
- Tag: Tag Free
- Molecular Weight: 12.7 kDa
- Purity: ≥95%, determined by SDS-PAGE.
- Endotoxin: ＜0.10 EU per 1 ug of the protein by the LAL method.
Quality
Solution protein.
Dissolved in sterile 20 mM sodium citrate(pH=2.5) bu_x001F_er .
This solution can be diluted into other aqueous bu_x001F_ers. Centrifuge the vial
prior to opening.
Formulation
Storage and Stability
Shipping Shipping with dry ice.
Components and Storage
Synonyms
12.7 kDa (monomer).
Accession #
Predicted Mol Mass
Source
P61812.
General Information
kDa NR R
Bioactivity
4 ug/lane protein was resolved
with SDS-PAGE under non-reducing (NR)
and reducing (R)conditions and
visualized by Coomassie blue staining.
HEK293
Ala303-Ser414.
SDS-PAGE
BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor
factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2;
TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2.
105
72
60
26
37
17
50
Activity Measured by the(CAGA)12-luciferase reporter assay.
The EC50 for this e_x001F_ect is 0.69ng/mL - UniProt/Swiss-Prot: P10600
- Available Sizes: 10 µg, 50 µg, 1 mg
Storage & Stability
Aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.
Quality Assurance
Each lot is tested for purity (SDS-PAGE, SEC-HPLC), endotoxin levels (LAL method), and bioactivity. A Certificate of Analysis (COA) is provided with every order.
Safety Data Sheets & Documentation
Protocols & Support
Reconstitution Protocol
1. Briefly centrifuge the vial to collect lyophilized powder at the bottom.
2. Reconstitute in sterile deionized water or PBS to a concentration of 0.2–1.0 mg/mL.
3. Gently pipette up and down to dissolve. Do not vortex.
4. Aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Download Data Sheet
Complete product data sheet including batch-specific QC data, sequence information, and storage guidelines.
Download PDF Data Sheet
