Mouse EPO Recombinant Protein (HEK293 Expression) — Tag Free
Price range: $198.00 through $5,600.00
HEK293 × Chaperone-Assisted Technology
Produced using our proprietary Z-Active® platform — HEK293 mammalian expression with chaperone-assisted folding for proper disulfide bonding, N-linked glycosylation, and 5–10× higher bioactivity vs Leading Suppliers.
Overview
Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of (EPO is required for biological activities in vivo (4). Mature mouse (EPO shares 95% amino acid sequence identity with rat (EPO and 73%-82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). (EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the (EPO receptor (EPO R) (8, 9). (EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of (EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating (EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce (EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). (EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).
Specifications
- Species: mouse
- Expression System: HEK293
- Sequence: Ala27-Arg192
- Tag: Tag Free
- Molecular Weight: 18.6 KDa
- Purity: > 95% by Tris-Bis PAGE;> 95% by SEC-HPLC
- Endotoxin: < 1 EU/μg of the protein by LAL method.
- UniProt/Swiss-Prot: P07321
- Available Sizes: 10 µg, 50 µg, 1 mg
Bioactivity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.4‑2 ng/mL.
Formulation & Reconstitution
Formulation: 0.22 μm filtered solution of PBS, pH 7.4.
Reconstitution: 0.2 mg/mL in sterile PBS, pH7.4
Storage & Stability
Aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.
Quality Assurance
Each lot is tested for purity (SDS-PAGE, SEC-HPLC), endotoxin levels (LAL method), and bioactivity. A Certificate of Analysis (COA) is provided with every order.
Safety Data Sheets & Documentation
Protocols & Support
Reconstitution Protocol
1. Briefly centrifuge the vial to collect lyophilized powder at the bottom.
2. Reconstitute in sterile deionized water or PBS to a concentration of 0.2–1.0 mg/mL.
3. Gently pipette up and down to dissolve. Do not vortex.
4. Aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Download Data Sheet
Complete product data sheet including batch-specific QC data, sequence information, and storage guidelines.
Download PDF Data Sheet
